И
иван
⚪
А у нас где-нибудь делают immunoglobulin M immunosorbent agglutination assay (ISAGA)?
Уверен, что нигде.
Уверен, что нигде.
Y
Yara
⚪
Здесь (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15916070&query_hl=4) неплохие результаты по ПЦР сэмплов амниотической жидкости.
M
maikl
⚪
Интересная статья с классификацией беременных при токсоплазмозе:
http://www.degruyter.com/journals/jpm/pdf/31_36.pdf#search='antiToxoIgG%20avidity (http://www.degruyter.com/journals/jpm/pdf/31_36.pdf#search='antiToxoIgG%20avidity)'
Mothers were classified according to the
classification system in immunocompetent pregnant
women as produced by the working party of
the European Research Network on Congenital
Toxoplasmosis [9].Thus categories of primary maternal
infection during pregnancy were:
• Definite: if evidence of seroconversion in pregnancy
or positive culture from maternal blood.
• Probable: if evidence of seroconversion within
two months before conception or significant
rise of IgG titers or high IgG titers, presence of
IgM and/or IgA and onset of lymphadenopathy
during pregnancy or high IgG titers and presence
of IgM and/or IgA in the second half of
pregnancy.
• Possible: if stable high IgG, without IgM, in the
second half of pregnancy or high IgG and presence
of IgM and /or IgA in the first half of pregnancy.
• Unlikely: if stable low IgG, with or without IgM
or stable high IgG, without IgM in early pregnancy.
• Not infected: if seronegative or maternal preconception
seropositive sample or positive IgM
and/or IgA without appearance of IgG.
The cut-off limits for IgG avidity were also em-
ployed to define the risk of vertical transmission.
Women were selected for further diagnostic work
if categorized as having definite, probable or possible
acute infection according to the European
Research Network and/or if IgG avidity was found
suggestive of recent infection. Patients were counseled
for amniocentesis and the criteria for amniotic
fluid sampling were as follows: first or early
second trimester as likely timing of seroconversion
and informed consent of the patient. Third
trimester referrals were advised to undergo combination
therapy without any sampling.Detection
of toxoplasma in amniotic fluid (AF) was carried
out with the use of in vitro culture and a PCR approach.
http://www.degruyter.com/journals/jpm/pdf/31_36.pdf#search='antiToxoIgG%20avidity (http://www.degruyter.com/journals/jpm/pdf/31_36.pdf#search='antiToxoIgG%20avidity)'
Mothers were classified according to the
classification system in immunocompetent pregnant
women as produced by the working party of
the European Research Network on Congenital
Toxoplasmosis [9].Thus categories of primary maternal
infection during pregnancy were:
• Definite: if evidence of seroconversion in pregnancy
or positive culture from maternal blood.
• Probable: if evidence of seroconversion within
two months before conception or significant
rise of IgG titers or high IgG titers, presence of
IgM and/or IgA and onset of lymphadenopathy
during pregnancy or high IgG titers and presence
of IgM and/or IgA in the second half of
pregnancy.
• Possible: if stable high IgG, without IgM, in the
second half of pregnancy or high IgG and presence
of IgM and /or IgA in the first half of pregnancy.
• Unlikely: if stable low IgG, with or without IgM
or stable high IgG, without IgM in early pregnancy.
• Not infected: if seronegative or maternal preconception
seropositive sample or positive IgM
and/or IgA without appearance of IgG.
The cut-off limits for IgG avidity were also em-
ployed to define the risk of vertical transmission.
Women were selected for further diagnostic work
if categorized as having definite, probable or possible
acute infection according to the European
Research Network and/or if IgG avidity was found
suggestive of recent infection. Patients were counseled
for amniocentesis and the criteria for amniotic
fluid sampling were as follows: first or early
second trimester as likely timing of seroconversion
and informed consent of the patient. Third
trimester referrals were advised to undergo combination
therapy without any sampling.Detection
of toxoplasma in amniotic fluid (AF) was carried
out with the use of in vitro culture and a PCR approach.
F
FSV
⚪
Am J Trop Med Hyg. 2004 Dec;71(6):831-5. Related Articles, Links
Detection of Toxoplasma gondii DNA and specific antibodies in high-risk pregnant women.
Nimri L, Pelloux H, Elkhatib L.
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan. nimri@just.edu.jo
Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission to the fetus. This study was conducted to test the utility of a polymerase chain reaction (PCR) assay to detect recent infections with Toxoplasma in pregnant women. One hundred forty-eight women with high-risk pregnancies who had abnormal pregnancy outcomes (cases) and 100 with normal pregnancies (controls) were tested for the presence of Toxoplasma DNA in their blood by a nested PCR and specific antibodies to Toxoplasma by an enzyme-linked immunosorbent assay. The IgG results of the cases differed significantly from those of the controls (54% and 12%, respectively; P < 0.02). Four (2.7%) of the cases were IgM positive, but none of the controls were positive. Detection of Toxoplasma DNA in 20 (8.1%) of the IgG-positive cases suggests a recent infection. The risk factors associated with the infection were eating raw meat and contact with soil. The diagnostic serology of recent infection in early pregnancy could be confirmed by a positive Toxoplasma-specific PCR result in blood samples collected in the first half of pregnancy, even in the presence of serologic results difficult to interpret due to the lack of sequential follow-up during pregnancy.
PMID: 15642979 [PubMed - indexed for MEDLINE]
Detection of Toxoplasma gondii DNA and specific antibodies in high-risk pregnant women.
Nimri L, Pelloux H, Elkhatib L.
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan. nimri@just.edu.jo
Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission to the fetus. This study was conducted to test the utility of a polymerase chain reaction (PCR) assay to detect recent infections with Toxoplasma in pregnant women. One hundred forty-eight women with high-risk pregnancies who had abnormal pregnancy outcomes (cases) and 100 with normal pregnancies (controls) were tested for the presence of Toxoplasma DNA in their blood by a nested PCR and specific antibodies to Toxoplasma by an enzyme-linked immunosorbent assay. The IgG results of the cases differed significantly from those of the controls (54% and 12%, respectively; P < 0.02). Four (2.7%) of the cases were IgM positive, but none of the controls were positive. Detection of Toxoplasma DNA in 20 (8.1%) of the IgG-positive cases suggests a recent infection. The risk factors associated with the infection were eating raw meat and contact with soil. The diagnostic serology of recent infection in early pregnancy could be confirmed by a positive Toxoplasma-specific PCR result in blood samples collected in the first half of pregnancy, even in the presence of serologic results difficult to interpret due to the lack of sequential follow-up during pregnancy.
PMID: 15642979 [PubMed - indexed for MEDLINE]
В
Виктор
⚪
Clin Microbiol Infect. 2004 Oct;10(10):937-9. Related Articles, Links
Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR.
Jalal S, Nord CE, Lappalainen M, Evengard B; ESCMID Study Group on Toxoplasmosis.
Karolinska Institutet, Department of Laboratory Medicine, Division of Clinical Bacteriology, Karolinska University Hospital, Huddinge, Sweden. shah.jalal@labmed.ki.se
DNA was extracted with a modified Qiagen DNA Mini Kit method from 20 clinical samples and was amplified by PCR using specific primers for the T. gondii B1 gene. T. gondii was detected correctly in 18 of the 20 clinical samples in < 5 h, with a detection limit of two parasites/sample. The results were in good agreement with those obtained by a more complicated and time-consuming procedure involving two-step nested PCR and either liquid hybridisation or colorimetric detection using internal probes.
PMID: 15373893 [PubMed - indexed for MEDLINE]
Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR.
Jalal S, Nord CE, Lappalainen M, Evengard B; ESCMID Study Group on Toxoplasmosis.
Karolinska Institutet, Department of Laboratory Medicine, Division of Clinical Bacteriology, Karolinska University Hospital, Huddinge, Sweden. shah.jalal@labmed.ki.se
DNA was extracted with a modified Qiagen DNA Mini Kit method from 20 clinical samples and was amplified by PCR using specific primers for the T. gondii B1 gene. T. gondii was detected correctly in 18 of the 20 clinical samples in < 5 h, with a detection limit of two parasites/sample. The results were in good agreement with those obtained by a more complicated and time-consuming procedure involving two-step nested PCR and either liquid hybridisation or colorimetric detection using internal probes.
PMID: 15373893 [PubMed - indexed for MEDLINE]
K
Kati
⚪
Здесь (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15305712&query_hl=4) пишут что:
The B1 PCR performed on blood samples was less sensitive than when it was performed on other biological fluids requiring 100 tachyzoites, instead of 10.
А другие (см. выше) пишут о высокой диагностической ценности ПЦР сыворотки крови матери в случае трудноинтерпретируемых результатов ИФА.
The B1 PCR performed on blood samples was less sensitive than when it was performed on other biological fluids requiring 100 tachyzoites, instead of 10.
А другие (см. выше) пишут о высокой диагностической ценности ПЦР сыворотки крови матери в случае трудноинтерпретируемых результатов ИФА.
G
Gulya
⚪
J Egypt Soc Parasitol. 2004 Aug;34(2):559-70. Related Articles, Links
Evaluation of semi-quantitative PCR and IgG & IgM ELISA in diagnosis of toxoplasmosis in females with miscarriage.
Abdel-Hameed DM, Hassanein O.
Department of Parasitology, and Clinical Pathology, Medical Research Center, Faculty of Medicine, Ain-Shams University, Cairo 11566, Egypt.
One hundred female (age 20-42 yrs) patients were classified into group I: 40 patients presented with abortion in the first trimester; group II: 33 patients with abortion in the second trimester and group III: 27 patients with intrauterine fetal death (IUFD). The positive percentages of semi-quantitative PCR and both IgG & IgM ELISA were 38% and 35% respectively. Ten (26.3%) cases out of 38 were positive for toxoplasmosis by both PCR and ELISA-IgG, while 5 (13.2%) cases out of 38 were positive by both PCR and ELISA-IgM, whereas 16 (42.1%) cases out of 38 PCR positive cases were positive by both ELISA IgG & IgM. Sensitivity and specificity of both ELISA IgG and IgM were 81.57% & 93.54% respectively. False negative by ELISA were found in 7 cases out of 38 positive toxoplasmosis cases detected by semi-quantitative PCR. Three cases out of the 7 cases with false negative by ELISA were detected with a trophozoite copy load of 10(1) trophozoite /mL in the blood sample by semi-quantitative PCR. So, the semi-quantitative PCR detected low levels of parasite DNA recommending its usefulness especially in the early stages of the disease when low amount of antibodies can't be detected by serological method or even by the conventional PCR.
Publication Types:
Evaluation Studies
PMID: 15287178 [PubMed - indexed for MEDLINE]
Здесь опять говорится о приоритете полуколичесвтенной ПЦР перед определением антител...
Конечно, нужно смотреть на дизайн исследования и полные тексты статей и многое зависит от метода ПЦР.
Вобщем я понял, что в Украине да и России ПЦР сыворотки крови пока не может быть рутинным исследованием.
В моей личной практике если пациентка обращается до беременности (а именно так я рекомендую), антитела на инфекции группы TORCH сдаются преконцепционно, что почти всегда позволяет избегать подобных казусов.
Evaluation of semi-quantitative PCR and IgG & IgM ELISA in diagnosis of toxoplasmosis in females with miscarriage.
Abdel-Hameed DM, Hassanein O.
Department of Parasitology, and Clinical Pathology, Medical Research Center, Faculty of Medicine, Ain-Shams University, Cairo 11566, Egypt.
One hundred female (age 20-42 yrs) patients were classified into group I: 40 patients presented with abortion in the first trimester; group II: 33 patients with abortion in the second trimester and group III: 27 patients with intrauterine fetal death (IUFD). The positive percentages of semi-quantitative PCR and both IgG & IgM ELISA were 38% and 35% respectively. Ten (26.3%) cases out of 38 were positive for toxoplasmosis by both PCR and ELISA-IgG, while 5 (13.2%) cases out of 38 were positive by both PCR and ELISA-IgM, whereas 16 (42.1%) cases out of 38 PCR positive cases were positive by both ELISA IgG & IgM. Sensitivity and specificity of both ELISA IgG and IgM were 81.57% & 93.54% respectively. False negative by ELISA were found in 7 cases out of 38 positive toxoplasmosis cases detected by semi-quantitative PCR. Three cases out of the 7 cases with false negative by ELISA were detected with a trophozoite copy load of 10(1) trophozoite /mL in the blood sample by semi-quantitative PCR. So, the semi-quantitative PCR detected low levels of parasite DNA recommending its usefulness especially in the early stages of the disease when low amount of antibodies can't be detected by serological method or even by the conventional PCR.
Publication Types:
Evaluation Studies
PMID: 15287178 [PubMed - indexed for MEDLINE]
Здесь опять говорится о приоритете полуколичесвтенной ПЦР перед определением антител...
Конечно, нужно смотреть на дизайн исследования и полные тексты статей и многое зависит от метода ПЦР.
Вобщем я понял, что в Украине да и России ПЦР сыворотки крови пока не может быть рутинным исследованием.
В моей личной практике если пациентка обращается до беременности (а именно так я рекомендую), антитела на инфекции группы TORCH сдаются преконцепционно, что почти всегда позволяет избегать подобных казусов.
M
maria naumova
⚪
Не совсем понятно, как они доказывали recent toxoplasmosis ? Однако многообещающая статья. Похоже, прогресс идет.
E
EGC
⚪
В моей личной практике если пациентка обращается до беременности (а именно так я рекомендую), антитела на инфекции группы TORCH сдаются преконцепционно, что почти всегда позволяет избегать подобных казусов.
Конечно, это решает много проблем, особенно в смысле краснухи и токсоплазмоза.
Недавно у нас был скрининг всех беременных на ТОРЧ. По уму бы его результаты обработать и при последующих беременностях учитывать для адекватного назначения тестов.
Пока стараюсь это делать у нас в центре, благо есть компъютерная база обследований и можно при опросе беременной поднять ее анализы. Однако это ведь бессистемные попытки. Кто-то консультируется, кто-то нет. Опять же база только нашего центра.
Конечно, это решает много проблем, особенно в смысле краснухи и токсоплазмоза.
Недавно у нас был скрининг всех беременных на ТОРЧ. По уму бы его результаты обработать и при последующих беременностях учитывать для адекватного назначения тестов.
Пока стараюсь это делать у нас в центре, благо есть компъютерная база обследований и можно при опросе беременной поднять ее анализы. Однако это ведь бессистемные попытки. Кто-то консультируется, кто-то нет. Опять же база только нашего центра.
A
ANNA_CORRECT
⚪
Спасибо за ответ. Попробуйте выяснить за дюфастон, хотя, ИМХО, это выдумка зав. лабораторией.
Она не только зав.лабораторией - она врач, которая комментирует результаты и видимо назначает лечение. Сложилось впечатление, что она в этом "бизнесе" давно и набрала статистически значимые результаты. Речь шла о десятках выявленных ею совпадениях ложноположительности IGm и приема дюфастона.
Наталья.
Она не только зав.лабораторией - она врач, которая комментирует результаты и видимо назначает лечение. Сложилось впечатление, что она в этом "бизнесе" давно и набрала статистически значимые результаты. Речь шла о десятках выявленных ею совпадениях ложноположительности IGm и приема дюфастона.
Наталья.
Е
елена07
⚪
Она не только зав.лабораторией - она врач, которая комментирует результаты и видимо назначает лечение. Сложилось впечатление, что она в этом "бизнесе" давно и набрала статистически значимые результаты. Речь шла о десятках выявленных ею совпадениях ложноположительности IGm и приема дюфастона.
Наталья.
Уважаемая Наталья,
данная тема была предназначена не для Вас, а для специалистов. Десятки, выявленные ею совпадений относятся к категории С (самой последней) по уровню доказательности. Опять же, ничего плохого не хочу сказать о зав. лабораторией, тем более заочно.
Наталья.
Уважаемая Наталья,
данная тема была предназначена не для Вас, а для специалистов. Десятки, выявленные ею совпадений относятся к категории С (самой последней) по уровню доказательности. Опять же, ничего плохого не хочу сказать о зав. лабораторией, тем более заочно.